GFP 抗体
Quick Overview for GFP 抗体 (ABIN289429)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应 (详细)
- Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml
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纯化方法
- Affinity chromatography purified
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过滤
- Filtered
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免疫原
- GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.
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应用备注
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ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
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: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).
: "Correlative GFP-immunoelectron microscopy in yeast." in: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).
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: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
抗原
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